The event is live or view on-demand:

Protein & DNA/RNA Structure Modeling for Cryo-EM maps

November 16, 2023 (Thursday)

New York (EST) Thursday, November 16th, 2023 from 11:00 AM - 12:00 PM.
California (PST) Thursday, November 16th, 2023 from 8:00 AM - 9:00 AM.
London Thursday, November 16th, 2023 from 4:00 PM - 5:00 PM.
China Friday, November 17th, 2023 from 12:00 AM - 1:00 AM.
Japan/Korea Friday, November 17th, 2023 from 1:00 AM - 2:00 AM.

Speakers:

Daisuke Kihara, Genki Terashi, & Xiao Wang, Purdue University

 

This webinar introduces software tools for protein and DNA/RNA structural modeling in cryo-EM maps: DeepMainmast for protein structure, CryoREAD for DNA/RNA, and DAQ for structure validation of protein structure models. Our speakers will introduce the algorithms of these tools and explain how to use them on web servers and other platforms. Whether you are new to structure modeling for cryo-EM maps or looking to enhance your skills, this webinar offers valuable insights into the latest developments and practical tools in structural modeling.

See references on the registration page for more information about the tools.

You will learn...

 1. The overview of the algorithm of computational modeling tools, DeepMainmast (for proteins) and CryoREAD (for DNA/RNA).
 2. The algorithm of the protein structure model quality estimation tool, the DAQ score.
 3. How to use the tools on the web server and Google Colab platform.

This 1-week in-person short course will be an immersive experience in the art and science of cryoEM held at the Simons Electron Microscopy Center housed at the New York Structural Biology Center in NYC, USA. The mornings offer lectures and stimulating round table discussions. The afternoons provide hands-on practicals to reinforce fundamental concepts and topics covered earlier in the day.
 

Lecturers include:
Joachim Frank (Columbia University)
Victor Chen & Wen Jian (Purdue University)
Fred Sigworth (Yale University)
Amedee des Georges & Reza Khayat (CUNY)
Rich Hite (Memorial Sloan Kettering Cancer Center)
Piliar Cossio (Flatiron Institute)
Jack Turner (EMBD/EMPIAR)
Oli Clarke (Columbia University)
Gira Bhabha & Damian Ekiert (New York University)

Dear Colleagues

We (the board members of the Pittsburgh Diffraction Society) are pleased to announce the availability of travel awards to attend the 80^th Pittsburgh Diffraction Conference. The Conference will be held at the Society’s ancestral home, in Pittsburgh, PA, on October 15, 2023 â€" October 17, 2023.

The awards are aimed at students, postdoctoral fellows as well as (non-faculty) staff scientists. Applications from minorities underrepresented in the sciences are especially encouraged. Details can be found here as well as attached.

The conference program includes

·        A historic overview on the Pittsburgh Diffraction Society

·        Scientific Sessions covering the Latest Development at Light Sources, High-pressure Applications, Structure-based drug design, Synergy of Computation and Crystallography, Crystallography and Cryo-EM, Structure property relationships in Quantum and Layered Materials, and many more!

·        A Young Investigator Platform showcasing achievements of students and postdocs

·        Student posters are eligible for the Chung Soo Yoo Award, which carries a $400 cash prize, related to materials and biological research, respectively

·        Fun social activities and Vendor Shows!

·        A Phenix Workshop prior to the conference (Oct 14, 2023, limited availability!)

Â

For the entire program, the venue and more details, and to register, please visit the conference web page.

Ana Casanal (Human Technopole, Milan, IT)
Michael Grange (Rosalind Franklin Institute, Oxford, UK)
Sebastian Klinge (Rockefeller University, New York, USA)
Alicia Michael (FMI Basel, CH/IST Austria, AT)
Arne Moeller (University of Osnabrück, DE)
Clemens Plaschka (IMP, Vienna, AT)
Dong Si (University of Washington Bothell, USA)
Guilia Zanetti (Birkbeck College, London, UK)
Tzviya Zeev-Ben Mordehai (Utrecht University, NL)

Registration fee:
100 Euro for PhDs/Postdocs Academic
200 Euro Group Leaders/Academic
300 Euro for Industry

Topics include Sample Preparation, Microscopy, Image Processing, Protein Dynamics and more.

The flagship of the European Crystallographic Association (ECA) activities is the European Crystallographic Meeting (ECM), held every year, except when there is an IUCr Congress. The ECMs are the main meeting point for crystallographers in the ECA area to show their recent research. Microsymposia at ECMs are proposed and organized by ECA SIGs.

ECM 34 will be organized at the Padova Congress Centre on behalf of ECA and AIC. It will include all topics of interest to crystallography and it will be organized in synergy with EPIDC 18, which will follow ECM 34 in the same location.

The Young Crystallographers are a working group within the German Society for Crystallography (DGK). For 10 years, we meet regularly in autumn at different universities or the STOE facilities.

These meetings specifically aim to provide a platform for networking and scientific exchange for young scientists with an interest in crystallography, as well as to establish contacts with industry and research institutions.
We want to give young scientists the opportunity to gain early experience in scientific presentation in a relaxed atmosphere and without the stress of a larger conference.

For our grand anniversary, we are happy to meet at the DESY facilities! We are working hard to allow you deep insights as well as a great overview of the possibilities at this large-scale facility.

During the 3-day meeting, you willÂ

• present your own work as a poster (A0 portrait) and lightning talk (~5 min.)
• have the DESY campus tour
• participate in an experiment and get a hands-on experience at a beamline of your choice at PETRA III (see The Experiments)
• hear academic talks from senior scientists
• hear industry talks from our sponsors/partners

At the end of the meeting, you will also have the chance to join a tour of the European XFEL Campus. If you intend to participate, please indicate so during registration. Note that you will need to get to the XFEL facilities by public transport as it is a bit outside the main DESY campus.

The registration fee of 60 € includes the tours and experiments at DESY, coffee breaks as well as lunch and BBQ on Thursday.

Deadline for registration is 22.08.23.

We are really happy to announce the first Grenoble Practical Workshop 
dedicated to helical symmetry determination of biological polymers 
imaged by cryo-EM, *17th to 21st of July 2023, online*.

Determining the helical parameters from a sample with helical 
symmetry(ies) is often the bottleneck for successful structure 
determination. The learning curve to get familiar with this process is 
steep and full of crevices.

In this workshop, we will guide the participants who already have 
helical assemblies data in their hands (see below) through this process, 
exploiting all possible information, including power spectra 
interpretation, 2D class-averages analysis and clue hunting from raw 
data inspection.

The practicals will cover the use of helixplorer 
(https://urldefense.com/v3/__https://rico.ibs.fr/helixplorer/__;!!Mih3wA!FuXAIKSush5n5zbFX1AKGJLHIyqhXwFf8VHpoYSSWGs8Pp3GEfVBZBiBYLlnaFEBrKTzfbA8VwHrMubwy_iaUharVpj2$ ), but will not be limited to this 
approach.


Although the main focus will be on helical parameters determination, we 
will also discuss general reconstruction strategies, including dealing 
with flexible helices, multiple symmetries, and merging of data from 
different symmetry filaments using subtraction approaches.

In order to apply to the workshop, applicants :

-can be at any career level (PhD, postdocs, senior scientists, ...)

-must have an ongoing project involving a helical sample, and have 
difficulties in solving its structure

-agree to work on their own data during the workshop. Participants are 
expected to share some raw images, 2D-class averages, and sum of PS with 
the organizers (and screen sharing with other participants). However, 
the name of their protein/organism and the exact nature and function of 
their protein/filaments will remain confidential.

Please send your application, including a short letter describing your 
project and difficulties, screenshots of raw data and 2D class-averages, to

ambroise.desfosses@ibs.fr

leandro.estrozi@ibs.fr

*Before the 15th of June. *

Lecture 27:  "Topological Analysis of the Electrostatic Potential in Molecular Systems" by Enrique Espinosa (Laboratoire de Cristallographie, Résonance Magnétique et Modélisations,  Université de Lorraine, Nancy, France)

 Lecture 28: "Breaking the Ice: Cryo-EM's Quest for Better Resolution and Quality" by Holger Stark (MPI for Multidisciplinary Sciences, Goettingen, Germany)

The 4th RICCEM will be held in hybrid format.

Conference topics:

• Structure of membrane proteins;
• Structure and functions of the transcription and translation apparatus of the cell;
• Structure of viruses and chaperonins;
• Molecular organization of cells and organelles, studied by subtomogram averaging;
• Advances in EM technology and image processing;
• Medical applications of cryo-EM.

CryoNET is a collaboration network among the main cryo-EM facilities and groups in the Nordic region and we organise annual international symposia since 2018. 

Monday, May 1, 2023, 8:00-9:30 AM (PDT)
 

Speakers:

Prof. Christopher O. Barnes

Talk Title: Structure-guided Design of Next-generation Immunotherapies against Emerging Coronaviruses
 

Dr. Peter Denes

Talk Title: Count Every Electron

This course will introduce high-resolution Cryo-EM to the structural biology research community. Tt will include image formation and three-dimensional reconstruction theories and available software, tutorials, and demonstrations for data collection and analysis.
The course is free of charge, but you will need to register: here

• MS1. Refinement and development of microscopy techniques
• MS2. Studies for structure and strain in materials
• MS3. Electron microscopy in-situ and in-operando techniques
• MS4. Functional materials
• MS5. Microscopy of soft and beam-sensitive materials
• MS6. Use of spectroscopic techniques in material science and geoscience

• LS1. Beyond the resolution revolution - new challenges in cryo-EM/ET
• LS2. Image analysis and data visualization
• LS3. Correlative light and electron microscopy
• LS4. Ultrastrucutral pathology and disease understanding
• LS5. Automated and AI based microscopy imaging and analysis
• LS6. In situ and live cell microscopy

This is a hybrid workshop hosted at Stanford-SLAC Cryo-EM Center (S2C2).

The workshop will alternate theoretical discussions with practical examples using experimental data with a main focus on Single Particle Analysis (SPA). We will demonstrate simultaneously how to apply different software packages at different stages of the image processing pipeline (like Relion, Xmipp, CryoSPARC, among others), a task that we will achieve transparently using the software integration framework Scipion. We will also address some advanced topics in Tomography in which an extra degree of robustness can be introduced along similar lines as those introduced in SPA with the help of Scipion runn
 ing iMod, EMAN, Relion, Dynamo, and Xmipp. Attendees are expected to be familiar with standard image processing pipelines in Single Particle Analysis. On the last day the talks on theory and practices will focus on advanced image processing topics, as well as their connection with molecular motion, 3D visualization, atomic modeling, structure validation, and drug design.

This will be a hybrid workshop. Due to available resources, we will have only 50 spots for onsite hands-on practical participation with all others attending via Zoom.

Â

Participants will be able to analyze their own data and tutorial
datasets using dedicated Amazon Web Services instances.

We will cover the following software packages:
-RELION (led by Liz Kellogg, Cornell Univ.)
-cryoDRGN (led by Ellen Zhong, Princeton Univ.)
-cryoSPARC (led by Structura Inc.)
-Model building in Coot/Phenix (led by Alan Brown, Harvard Medical School)