The course is aimed at early-career researchers and facility staff with experience in cryo-EM who are interested in expanding their practical and theoretical knowledge of cryo-FIB/SEM and cryo-ET. The program includes focused lectures and intensive hands-on sessions covering:
 

• Sample vitrification and lamella thinningÂ
• Plasma FIB milling and cryo-lift-outÂ
• Tomographic data acquisition and analysis

This workshop will bring together leading experts to share the latest best practices, tools, and techniques in cryoEM model building, refinement, visualization, validation, and deposition.
Confirmed speakers include:

 * Paul Emsley & Lucrezia Catapano (MRC)

 * Dari Kimanius (Chan Zuckerberg Institute for Advanced Biological Imaging)

 * Rachael Kretsch (Stanford University)

 * Stephanie Wankowicz (Vanderbilt University)

 * Tom Goddard (UCSF)

 * Tristan Kroll (Altos Labs)

 * Nigel Moriarty & Oleg Sobolev (LBNL)

 * Jane Richardson (Duke University)

 * Greg Pintilie (Stanford University)

 * Stephen Burley & Justin Flatt (RCSB Protein Data Bank)
The program will feature expert-led talks and live demonstrations on Coot, Phenix, ModelAngelo, ISOLDE, ChimeraX, and other software tools, along with discussions on structure validation and deposition guidance. All sessions will be recorded and made available on the S2C2 website after the event for participants to review or catch up on missed content.

Hosted by the Stanford-SLAC CryoEM Center (S2C2), his workshop will bring together leading experts to share the latest best practices, tools, and techniques in cryoEM model building, refinement, visualization, validation, and deposition.
Confirmed speakers include:
 

Paul Emsley & Lucrezia Catapano (MRC)
 *
Dari Kimanius (Chan Zuckerberg Institute for Advanced Biological Imaging)
 *
Rachael Kretsch (Stanford University)
 *
Stephanie Wankowicz (Vanderbilt University)
 *
Tom Goddard (UCSF)
 *
Tristan Kroll (Altos Labs)
 *
Nigel Moriarty & Oleg Sobolev (LBNL)
 *
Jane Richardson (Duke University)
 *
Greg Pintilie (Stanford University)
 *
Stephen Burley & Justin Flatt (RCSB Protein Data Bank)
 

The program will feature expert-led talks and live demonstrations on Coot, Phenix, ModelAngelo, ISOLDE, ChimeraX, and other software tools, along with discussions on structure validation and deposition guidance. All sessions will be recorded and made available on the S2C2 website after the event for participants to review or catch up on missed content.

 https://s2c2.slac.stanford.edu/form/stanford-slac-cryoem-center-mode

This course is designed for students and postdocs interested in using cryo-ET and cryo-FIB for their research. It includes lectures, demonstrations, and hands-on practicals covering sample preparation, FIB milling, lamella preparation, data collection, processing and more.

We have a limited number of spaces available to selected members of the academic community. Places will be allocated on the grounds of merit and anticipated benefit.

This in-person beginners? course is aimed at PhD students and postdoctoral researchers who are new to cryo-EM and plan to incorporate it into their research. The programme will combine core theoretical lectures with practical training in single-particle cryo-EM, covering:

 * Negative stain grid preparation and screening
 * Cryo-EM sample preparation (including sample preparation for time-resolved cryo-EM)
 * Grid screening and data collection using EPU on a Titan Krios microscope
 * Demonstration of single-particle data processing using RELION

2025 Pittsburgh Diffraction Conference (PDC)

Hosted by Brookhaven National Laboratory
October 9â€"12, 2025

On behalf of the Conference Committee, we are delighted to invite you to 82nd annual Pittsburgh Diffraction Conference (PDC). This year’s conference will be held October 9â€"12, 2025 at Brookhaven National Laboratory, on New York’s beautiful Long Island.

The Pittsburgh Diffraction Conference is one of the longest-running scientific meetings in North America focused on the advancement of structural science through diffraction-based methods. Bridging both life sciences and materials sciences, the conference brings together researchers who utilize laboratory-based instruments, X-ray light sources, electron diffraction, and neutron scattering to explore the structure and dynamics of matter. It serves as a vibrant forum for sharing recent scientific breakthroughs, fostering interdisciplinary collaborations, and highlighting innovations across experimental and computational frontiers

This year’s program features a diverse and timely selection of breakout sessions, reflecting key areas of growth in the field. Topics include energy storage, emphasizing structural insights that enable next-generation energy technologies; AI and its applications in the development of emergent materials; novel materials with transformative properties; and the effects of extreme environments on structures, reveling details on dynamic processes and molecular motion that lead to biomolecular functions and a better understanding of complex soft materials.

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Conference Web Page and Registration:Â Â

https://www.bnl.gov/pittsburgh-diffraction-conference/index.phpÂ

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Important dates:

April 21, 2025Â Â Â Â Â Â Â Â General registration opens
July 31, 2025Â Â Â Â Â Â Â Â Deadline to submit an abstract for an oral/poster presentation
September 19, 2025Â Â Â General registration closes

Pre-event workshops:

PDF 101: A Hands on Tutorial to Total Scattering Methods (organizers: Milinda Abeykoon, Daniel Olds)

Hands-on Workshop: Crystal Clear Insights: Mastering Protein Crystallization (organizers: Crissy Traver, Vivian Stojanoff)

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Evening Events:

Brookhaven National Lab facility tour.
Friday, October 10, 2025
https://www.bnl.gov/pittsburgh-diffraction-conference/nsls2tour.php

Banquet Dinner
Danfords Hotel and Marina
25 E Broadway, Port Jefferson, NY 11777
Date: Friday, October 10, 2025, 7-9 p.m.

Awards Dinner
Charles B. Wang Center
100 Nicolls Road, Stony Brook, NY 11794
Date: Saturday, October 11, 2025, 7-9 p.m.

The third edition of the Grenoble practical workshop focused on the determination of helical symmetry in biological polymers imaged by cryo-EM will take place online from 
July 21st to 25th, 2025.
Ed Egelman and Arjen Jakobi will be speakers, whose expertise will greatly enrich both our discussions and hands-on sessions.

While *Helixplorer* (https://urldefense.com/v3/__https://rico.ibs.fr/helixplorer/__;!!Mih3wA!E8k6u4PapE61RoHy1fNa_JmGof9e65JNpZs2Dcf7d1ogbvN9gxduVMX-fpPxMI-nzq_qTqbseDr52Jf6kB_Jv66N9Rjr$ ) will play a central role in our practical sessions, we also encourage participants 
to explore alternative approaches to address a variety of research needs.

Beyond helical parameter determination, we will also explore `broader 
reconstruction strategies', including managing flexible helices, working 
with multiple symmetries, and integrating data from different filament 
symmetries using subtraction techniques.

We welcome applicants at all career stages (PhD students, postdocs, 
senior scientists, and beyond) who are currently working on helical 
samples and facing structural analysis challenges. During the workshop, 
participants will use their own data, sharing selected raw images, 2D 
class averages, and power spectra with the organizers. The specific 
identity and function of your protein or filament will remain confidential.

To apply, please email your application?including a brief description of 
your project and the difficulties you?re encountering, as well as 
screenshots of your raw data and 2D class averages with the 
corresponding *pixel size*? to:

 *ambroise.desfosses@ibs.fr*

 *leandro.estrozi@ibs.fr*

*Application deadline: June 10th.*

Invited speakers:

 * Aleksandar Antanasijevic, Ecole polytechnique federale de Lausanne, Switzerland
 * Petr Chlanda, Heidelberg University, Germany
 * Irina Gutsche, Institut de Biologie Structurale, Grenoble, France
 * Ohad Medalia, University of Zurich, Switzerland
 * Christopher Russo, MRC Laboratory of Molecular Biology, Cambridge, UK
 * Veijo Salo, The European Molecular Biology Laboratory, Heidelberg, Germany
 * Itziar Serna Mart?n, Thermo Fisher Scientific, Netherlands
 * Sebastian Tacke, Max Planck Institute of Molecular Physiology, Dortmund, Germany
 * Ilaria Testa, KTH Royal Institute of Technology, Stockholm, Sweden
 * Sonja Welsch, Max Plack Institute of Biophysics, Frankfurt, Germany
 * Xiaodong Zou, Stockholm University, Sweden

cryoNET speakers:

 * Mira Dombi, University of Copenhagen, Denmark
 * Max Renner, Umea University, Sweden
 * Klara Theresa Scholtissek, Aarhus University, Denmark
 * Sebastian Valenzuela, University of Gothenburg, Sweden

Join us for the first edition of FrostByte, a meeting for computational cryo-EM methods developers in the San Francisco Bay Area.

This is an event for the Bay Area cryo-EM methods community to connect, share practical insights, and discuss day-to-day challenges. While we welcome attendees from all backgrounds and roles, the focus will be on technical discussions among those directly engaged with computational methods in their daily work.

The workshop will offer a synergistic combination of lectures and practical hands-on sessions supported by leading experts in the field, providing the participants with the unique opportunity to establish a broad network with international specialists in the field.

This course will enable the participants to: prepare thick biological specimen using high-pressure freezing for on-grid lamella preparation (waffle method), implement cryo-CLEM correlative workflows for 3D targeting, set up automated lamella preparation, and perform cryo-volume imaging data acquisition.

We bring together experts at the intersection of microscopy and mass spec, topics include soft landing, holography, cryoEM, AFM, native MS, top-down proteomics and various single molecule imaging and mass spectrometry techniques to characterize (biological) macromolecules.

Course Objectives

• To provide training in state-of-the-art techniques for high resolution structure determination by cryoEM, including hands-on practical training in latest software suites for image processing
• Discuss data collection strategies
• Training in basic principles of structure determination using cryoEM
• Develop problem solving skills through discussions and practical sessions in the presence of international experts in the field
• Networking with experts, software developers, and researchers in cryoEM and learn not only the latest but also upcoming developments