This will be a hands-on workshop on the new software tool Fluctuating Finite Element Analysis (FFEA), which calculates dynamic trajectories for flexible biomolecules directly from cryo-EM/ET density maps. FFEA aims to provide an equivalent simulation tool for the EMDB as atomistic MD simulations have for the PDB. This approach uniquely uses volumetric meshes rather than atomistic co-ordinates as input such that it can model protein dynamics, protein-protein interactions, conformational switching between known configurational states and can be mapped back to atomistic co-ordinates, where these are available. This workshop is ideal for biomolecular simulators who would like to make full use of cryo-EM data in their modelling, or for experimentalists who would like to gain new theoretical insight into the dynamic behaviour of the structures they observe.

Registration: https://eventbooking.stfc.ac.uk/news-events/ffea-practical-workshop-381

Uniting NMR and Computational Approaches to Push the Frontiers of Dynamic Structure and Conformational Transitions Critical to Function and Dysfunction

Bio-Graphic Chemistry: Stories of Molecules that Shape Human Biology

Topics

• X-ray crystallography
• Electron microscopy
• Modelling, bridging scales to systems biology
• Structural biology in health and disease
• Radiation - photons, neutrons and electrons

Topics

• Recent advances in genomics, proteomics, and metabolomics
• Integrating different 'omics data
• Post-translational modification: expanding proteome diversity
• Novel 'omics technologies - including 'omics and structural biology
• Clinical applications of multi-omics

Topics

• Nucleosome Structure
• Recognition and Enzymatic Activity
• Chromatin Remodeling
• Nucelosome Diversity
• Higher Order Chromatin
• Genome Organisation
• Translational Applications

• Introduction to absorbance, fluorescence and circular dichroism spectroscopy
• Principles of CD spectroscopy
• Linear dichroism
• Good practice
• Data bases for biomacromolecules
• CD of membrane proteins
• What is inside a CD spectrometer
• DNA-metalcomplex interactions
• Applications of SRCD and CD to structural and functional genomics
• CD theory & Dichrocalc

MacCHESS is offering its seventh intensive HOWTO course in BioSAXS. Students will have a day and a half of lectures and hands-on software tutorials on the basics of BioSAXS data collection and processing from expert practitioners in the field. This will be followed by real data collection on MacCHESS beamlines (F1 and G1 stations).

While the primary purpose of the course is educational, students may bring a limited number of their own research samples. Prepared standard protein samples will be available to all students for practice.

Remote students are welcome, and will receive digital copies of all lectures, tutorials, and sample data, and will have access to a live stream of the course lectures on the first day. Due to the hands-on nature of the sessions, remote students will not have access to instructors during the software tutorial (though they will receive the tutorial documents). They also will not have data collection time during the course.

Course topics:

• Basic principles and processing methods
• Critical sample preparation and data collection procedures
• Evaluating data quality
• Computing envelopes and data modeling
• Online size-exclusion chromatography (SEC-SAXS)
• Overview of advanced and emerging methods
• What you need to know for publication
• Hands-on tutorial with data processing software

Students will be assigned into small groups for 6 hour data collection slots starting Wednesday afternoon and running through Friday morning. If you are coming as a group or if you need a particular time slot (due to early departing flight), please notify Richard Gillilan (reg8@cornell.edu) for scheduling. Lectures and tutorials are finished by 11:30am on Wednesday. There are no other officially scheduled activities besides your data collection slot, so you may wish to make your travel arrangements once you know when your slot will be.

SEC-SAXS will be available to students during the workshop. However, due to the time intensive nature of the technique, not everyone will be able to use it. The use of the SEC-SAXS setup will be prioritized for students/groups with appropriate samples. If you think your sample may benefit from SEC-SAXS, please notify Richard Gillilan (reg8@cornell.edu) for scheduling.

Polyamine Metabolism in Disease and Polyamine-Targeted Therapies

From Structure and Dynamics to Energy Landscapes and Personalized Medicine

New Modalities, Methods and Mechanisms for Chemical Biology

Exploring and Harnessing the Unique Properties of Nucleic Acids and Their Components

Membrane Protein Structure, Function and Dynamics: New Ways to Answer Old Questions

Intracellular Calcium Signals: Generation, Function and Therapeutic Intervention