Meeting participants working in protein crystallography and practitioners of cryo electron microscopy, tomography, and diffraction will have the opportunity to learn first-hand from internationally recognized experts contributing to the "Resolution Revolution". Recent advances in direct electron detection, automated cryogenic sample handling, phase plate technologies, and correlative cryo fluorescent light/electron microscopy hold considerable promise for protein crystallographers intent on studying larger, more complex, and often heterogeneous (conformational and compositional) assemblies of biological macromolecules that resist crystallization. At the close of the symposium, Invited Speakers will be asked to contribute to a round table discussion of the important roles that current and would-be cryo-EM/ET/ED practitioners can play in the future of the American Crystallographic Association.

May 6â€"7, 2017: Optional Pre-Course
May 8â€"12, 2017: Work­shop

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Con­tent Pre-Course

(Optional for expe­ri­enced par­tic­i­pants)

• Intro­duc­tion to elec­tron tomog­ra­phy and Seri­alEM
• Linux
• Data pro­cess­ing with IMOD and eTomo

Con­tent Work­shop

• Immer­sion freez­ing for cryo-electron tomog­ra­phy
• High pres­sure freez­ing and CEMO­VIS
• Cryo-CLEM
• Low-dose data col­lec­tion with Seri­alEM
• Direct elec­tron detec­tors
• Cryo-STEM tomog­ra­phy
• Pro­cess­ing of low-dose tilt series with IMOD
• Mod­el­ling and inter­pre­ta­tion of cryo-electron tomog­ra­phy data
• Subto­mo­gram aver­ag­ing with PEET

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The purpose of the workshop is to bring together the best scientists from the United Arab Emirates (UAE) and synchrotron experts and see how the most strategic fields for the UAEÂ can be addressed through synchrotron science. UAE scientists will include both national and non-national speakers. International top synchrotron experts will give a talk at the event.

One of the main points will be to understand what the requirements of the UAE scientists are in terms of synchrotron research (what types of beamlines, techniques, etc.). Once these are defined, we will try to understand what would be the best option in terms of costs and benefits for the UAE.

Michael Zachmann, Cornell University: New applications in material science enabled by Cryo-FIB lift-out and cryo STEM

Morgan Beeby, Imperial College London: High-throughput cryo-tomography using a side-entry cryoholder: insights into molecular evolution and function

Tecnobyte 1 - LOT - Recent Developments in Direct Detection Cameras for
Cryo-EM
Tecnobyte 2 - Linkam - A novel approach to plunge freezing and automated
sample handling
Tecnobyte 3 - JEOL Title TBC

Dan Claire, eBIC- Diamond Light: Data collection at the electron Bio-Imaging Centre (eBIC)

Freeze Frame Competition
Lunch
CMG AGM meeting

Deryck Mills, MPI for Biophysic: Cryo-EM: My journey to the final Frontier

Martha Briceno de Gutierez, Johnson Matthey: Practical applications of
Cryo Microscopy in Industry

This workshop precedes the Lorne Proteins meeting.

The workshop will include hands-on practicals in addition toproblem-solving-oriented talks from leading software developers in the field, including

• Steven Ludtke, lead developer of the popular, all-purpose EMAN2 suite of programs
• Hans Elmlund, lead developer of the SIMPLE/PRIME suite of programs for ab initio 3Dreconstruction
• Jose Miguel de la Rosa Trevin and Roberto Marabini, developers of the integrative software tool Scipion

The workshop will cover all computational aspects of single-particleanalysis, including 2D analysis, 3D starting model generation,heterogeneity analysis, and high-resolution refinement. You will beintroduced to and get the opportunity to try out the latest developments inthe field.

The meeting will accept 40 students.

• Channels
• Cryo-EM
• Dynamics
• Emerging techniques
• Enzymes
• Functional characterization
• Transporters

PURPOSE OF THE COURSE

Over the last 50 years, crystallography has developed from a method capable of determining the structures of isolated, soluble proteins to one able to provide detailed information on mechanisms of action of integral membrane proteins, whole viruses and the complex nano-machines that are central to cellular function. To discover how biology works researchers are now combining the power of crystallography with multiple other methods, spanning from the atomic to cellular scale, and including revolutionary developments in electron cryo-microscopy and tomography. This Course will celebrate its milestone as the 50th in the crystallography series started by Dorothy Hodgkin by focusing on integration:

1. of different techniques,
2. of molecular and cellular approaches and
3. of the crystallographic community, including diversity.

discussion of topics ranging from sample preparation to data analysis software. To commemorate the achievements of the Erice crystallography school over the past 50 years, the course will feature several sessions that reflect on the past and look to the future to highlight the factors that create an inclusive discipline.

APPLICATIONS

Interested candidates should register by 30th November 2016 using the form available in the school website (crystalerice.org).
Applicants may be able to apply for partial financial support. Please visit cristalerice to view the full eligibility criteria.

CURRENT LIST OF SPEAKERS AND TOPICS

• Nenad Ban (ETH Zurich):Â Protein synthesis by large cellular assemblies
• David Barford (LMB Cambridge): The anaphase-promoting complex
• Pamela Bjorkman (Caltec):Â Â Immune recognition of viral pathogens
• Tom Blundell (Cambridge University):Â Â TBD and "Celebrating the 50th International School of Crystallography"
• Bridget Carragher (NYSBC):Â Intro to Cryo-EM
• Wah Chiu (Baylor College of Medicine):Â Electron cryo-microscopy of biological nanomachines
• Elena Conti (MPI for Biochemistry):Â RNA metabolism
• Piet Gros (Utrecht University): The complement system
• Sun Hur (Harvard University):Â Signal transduction by immune receptors
• Yvonne Jones (University of Oxford):Â Cell surface signaling assemblies
• Werner Kühlbrandt (MPI for Biophysics): Membrane proteins: Structure and mechanisms
• John Kuriyan, (UC Berkeley):Â Cellular signal transduction
• Debora Marks(Harvard University):Â TBD
• Jenny Martin (Griffith University):Â Antimicrobial drug design
• Naoko Mizuno (MPI for Biochemistry):Â TBD
• Poul Nissen (Aarhus University):Â Membrane transporters in the brain
• Lori Passmore (LMB Cambridge):Â TBD
• Michael Rossmann (Purdue University):Â The structure of the Zika virus
• Michael Rout (The Rockefeller University:Â The nuclear pore complex
• Giovanna Scapin (Merck & Co):Â Â Structural Biology in Drug Discovery
• Irmi Sinning (Heidelberg University):Â Protein targeting and membrane protein biogenesis
• Titia Sixma (NKI Amsterdam):Â Molecular mechanisms in carcinogenesis
• David Stuart (University of Oxford):Â Virus structure and vaccine design
• Sriram Subramaniam (NCI Bethesda):Â Â Â High resolution electron microscopy
• Alessandro Vannini (ICR London):Â Â TBD
• Antoine van Oijnen (University of Wollongong):Â Single molecule biophysics
• Wei Yang (NIDDK Bethesda):Â Â Â Â DNA repair, replication and recombination
• Xiaodong Zhang (Imperial College London):Â Â Â DNA processing and damage response

WORKSHOPS

• Riccardo Pellarin (Pasteur Institute): Integrative Modeling Platform
• Cornelius Gati (MRC): RELION
• Randy Read (MRC): Validation/errors
• Paul Emsley (MRC) :COOT
• Debbie Marks & Chris Sanders (Harvard): EV fold
• Airlie McCoy (MRC): Solution and refinement of large complexes/low resolution systems
• Ardan Patwardhan (EBI/PDBe): EMDB/EMPIAR:Â linking structural information from molecules to cells

Invited Speakers

Jenny Hinshaw (NIDDK, Bethesda)

Georgios Skiniotis (University of Michigan, Ann Arbor)

Wen Jiang (Purdue University, Lafayette)

Hosted by CCP-EM and the Astbury Biostructure Laboratory (University of Leeds, UK) this one day workshop will focus on the RELION software package and is led by its developer Sjors Scheres (MRC-LMB).

The training will involve a hands-on tutorial of RELION 2.0 for cryo-EM single-particle analysis (http://www2.mrc-lmb.cam.ac.uk/relion). It will cover the entire process from initial micrograph inspection, CTF estimation, (automated) particle picking, 2D and 3D classification, 3D high-resolution refinement, movie-processing, and map sharpening.

Computational facilities will be provided along with refreshments.

Registration is free but not a guarantee of acceptance. Application for registration will close on Monday 15th August 2016, participants will be selected and we will confirm places no later than Friday 19th August 2016. There are 30 places at the workshop.

To address the recent surge of interest in the general biophysical community, the Cryo-EM Subgroup was initiated at the 2016 meeting with a program of speakers that were
selected from submitted abstracts. For the 2017 meeting, we have invited
the following speakers:

• Gira Bhabha, Univ. of California, San Francisco
• Eric Gouaux, Vollum Institute, Oregon Health & ScienceUniv
• Pascal Krotee (David Eisenberg Lab), Univ. of California, Los Angeles
• Rod MacKinnon, Rockefeller Univ.
• Stefan Raunser, Max Planck Inst of Molecular Physiology, Dortmund
• Kliment Verba (David Agard Lab), Univ. of California, San Francisco

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MicroED is a cryo-EM method that uses electron diffraction to facilitate structure determination from sub-micron sized 3D crystals of biological material. MicroED uses equipment and procedures that are standard on many cryo-EM:s, and because of this the technique can be easily adopted in labs and facilities around the world. To encourage the widespread use of MicroED we are offering this workshop to teach microscopists how to perform the technique and adopt it at their own institutions. The first MicroED workshop was held in February of 2016 and was heavily oversubscribed. For this reason we are offering a second workshop.

There is no registration fee for participation, and Janelia will provide on-site housing and meals. Attendees are expected to cover their own travel expenses and must be able to stay for the duration.

Space in the course is limited. The application deadline is August 1, 2016 (11:59 PM EDT)

The workshop will provide hands-on training on practical aspects of 2D crystal data collection on a Titan Krios, and cover in depth the theoretical foundations and the practical image processing of 2D crystal cryo-EM images with the 2DX software package. No previous experience is required.

Topics include:

Basics of 2D electron crystallography

• Introduction to 2D electron crystallography
• Cryo-electron microscopy sample preparation for 2D crystals of membrane proteins
• Cryo-EM data collection of 2D crystals
• Introduction to 2DX
• Drift correction using ZORRO Â

Basic image processing with 2DX

• Determination of the defocus, crystal tilt geometry, crystal lattice
• Crystal Unbending - including movie-mode drift correction of individual unit cells in dose-fractionated image series
• 2D merging and 3D merging with reconstruction via weighted back projection
• Automation of the processing pipeline

Advanced topics in image processing with 2DX

• Approaches for a partial reconstruction of amplitudes and phases inside of the missing cone
• Processing of 2D crystal unit cells with RELION and FREALIGN, under exploitation of neighborhood correlation of certain image parameters
• Advanced usage of the 2DX backend library
• Preparation of final results with quantitative description of statistical values (e.g., resolution) for export to databases (EMDB).

Computing infrastructure will be provided in place and participants are welcome to bring their own data sets and/or computer (OSX or Linux).

For registration, please include information about your affiliation, and a short motivation statement (max 200 words).
The registration fee is 250 CHF (travel and accommodation is not included), registration deadline is June 30th.

An Inaugural Symposium at UT Southwestern

Distinguished guest speakers include the cryo-electron microscopy and tomography experts Yifan Cheng (UCSF), Wah Chiu (Baylor College of Medicine), Nikolaus Grigorieff (Janelia Research Campus) and Dick McIntosh (University of Colorado, Boulder).

The GIAN-CEM3DIP practical course will cover :

• 3D Electron Microscopy: History and Basic Principles
• Biological sample and specimen preparation methods for TEM
• TEM- principles, image formation , particle detection, Fourier Transform, Convolution, CTF, PSF, Resolution and FSC
• Image processing, Point group symmetries, Classification, Orientation and Heterogenity of particles
• Getting the first model using Conical Tilt and Projection Matching
• Single particle CryoEM and 3D reconstruction
• Challenges of in-situ structural biology
• Principles and applications of Cryo Tomography. Cellular Tomography and Subtomogram averaging.
• Refinement of atomic models against CryoEM maps, Flexible fitting, Validation, Assessment and Data deposition.
• Hybrid methods: Protein Crystallography, Computational methods and CryoEM