A workshop of the Chinese 3DEM community

To meet the current and future needs of CryoEM technology, during this workshop, the following topics will be focused on:

       1. Novel algorithms to analyze molecular machineries with continuous conformational change.
       2. Image processing tools to analyze the structure of molecular machineries in situ.
       3. Tools and methods to aid accurate modeling based on CryoEM maps.
       4. EMDB/EMPIAR usage and deposition.

       The following speakers and teachers are invited to talk about the above topics:

       1. Abbas Ourmazd (University of Wisconsin-Milwaukee, USA)
       2. Slavica Jonic (IMPMC, Sorbonne University, France)
       3. Florian Schur (EMBL, Germany)
       4. Sai Li (University of Oxford, UK)
       5. Brendon Frenz (University of Washington, USA)
       6. Ardan Patwardhan (EMBL-EBI, UK)

       The workshop will last four days with intensive programs including courses in the morning, practices in the afternoon and free discussions in the evening. The approximate number of attendees for the courses will be 100 and the number for the practice session is restricted to 25. To be noted that there will be a video session for those who are not selected for practice session to watch the practice via a web-video and do practice using their own laptop.

       Graduate students, postdocs and young scientists who currently work in the CryoEM field with preliminary CryoEM image processing knowledge are particularly encouraged to attend this workshop.

Workshops will be held on the topics of:

• Membrane proteins and the methods of studying their structure;
• The structure and functions of the cells transcription apparatus;
• Subnanometer-resolution virus structures;
• Molecular organization of cells and organelles;
• New methods of sample preparation and data processing in cryo-electron microscopy.

 Keynote lectures:

 Prof Wolfgang Baumeister (Max-Planck-Institut für Biochemie, Germany)

 Dr Alexander Sobolevsky (Columbia University, NY, USA)

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Lomonosov Moscow State University will issue invitations for Russian visas for those who need them.

A recreation program that will take place after the Conference, including a visit to the Kremlin and Red Square in Moscow.

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Introducing the new Cryo-Electron Microscopy Platform for Structural Biology

The aim of this symposium is to promote the exciting opportunities in
structural biology opened by the advances in cryo-electron microscopy
and by the integration of structural biology approaches at multi-scale
level.

This symposium will celebrate the establishment of a new cryo-EM
platform at the EPN campus. The platform will offer access to a Titan
Krios microscope, which will operate as a user facility at the ESRF and
will reinforce the ongoing EM activity at the IBS and at the EMBL. The
new facility will be open to the international ESRF user community based on
peer-review of scientific merit and technical feasibility of their
projects. Scientists from ESRF, IBS and EMBL will support user
operation.

The symposium will bring together leading experts, junior researchers
and representatives of industrial partners to present and discuss the
latest developments and the future trends in cryo-EM as well as their
applications in addressing biological challenges.

We encourage participants to present their scientific results in a
poster session, with the possibility of being selected for a short talk.
Due to limited capacity (maximum of 80 external participants), early
registration is encouraged.

• Micrograph movie alignment
• CTF estimation
• 2D classification
• 3D starting model generation
• High-resolution 3D refinement
• 3D focused classification and heterogeneity analysis

For the past several years a number of EM labs in the larger Bay Area have held a bi-annual day long meeting to discuss our research and the latest developments in cryo-EM methods. The next meeting will be held on Wednesday, March 29th at UC Santa Cruz. We invite participation by members the EM community who are interested in joining us.Â

Please contact Melissa Jurica for further details and to RSVP.

Exploring the Complexity of Life by Cryo-EM

The first international cryo-EM symposium in Sweden will take place in Stockholm (May 8) and Umeå (May 9), and present the developments that are shaping this scientific breakthrough. Topics covered include central discoveries in cryo-EM, the most recent methodological developments and applications to central biological questions.

May 8 (Stockholm)

• New frontiers in single-particle cryo-EM: imaging short-lived states and tracking a continuum of states
  Joachim Frank, Columbia University, United States
• Atomic resolution cryo-EM Sriram Subramaniam, National Institute of Health, United States
• A molecular movie of the exitation-contraction coupling
  Nieng Yan, Tsinghua University, China
• Mitochondrial membrane proteins and the resolution revolution in cryo-EM
  Werner Kühlbrandt, MPI Biophysics, Germany
• Single particle analysis of small molecules with the Volta phase plate
  Radostin Danev, MPI Biochemistry, Germany
• Next steps for automation in cryo-EM
  Bridget Carragher, NYSBC, United States
• Cryo-EM methods that drive the revolution: example applications in Alzheimer’s disease
  Sjors Scheres, MRC-LMB, United Kingdom
• Future software development challenges in cryo-EM
  Jose Miguel de la Rosa Trevin, Cryo-EM Facility, Sweden

May 9 (Umeå)

• Reducing the cost and increasing the speed of cryoEM
  Richard Henderson, MRC Laboratory of Molecular Biology, Cambridge, UK
• Structures of scarce native complexes by cryo-EM: Finally following eukaryotic ribosome biogenesis at molecular resolution
  Roland Beckmann, Gene Center, University of Munich LMU, Germany
• Single particle analysis of chromatin complexes
  Sara Sandin, Nanyang Technological University, NTU, Singapore
• Protein synthesis in organelles at cryo-EMÂ resolution
  Alexey Amunts, Stockholm University
• Opening windows into the cell:  Revealing the molecular  architecture of the nuclear periphery
  Elizabeth Villa, University of California, San Diego, USA
• A Hitchhiker’s guide to cryo-tomography: travel tips on things to do
  Elisabeth Fischer, National Institute of Health, Hamilton, MT, USA
• Using new methods in cryo-EM to gain mechanistic insight into gene expression
  Lori Passmore, MRC Laboratory of Molecular Biology, Cambridge, UK
• Enabling and doing structural biology in situ â€" Recent advances in cryo-electron tomography
  Jürgen Plitzko, Max Planck Institute of Biochemistry, Martinsried/Munich, Germany

Opening New Frontiers with Cryoelectron Microscopy

The instructors for the workshop will include: John Briggs (EMBL), Anchi Cheng (TSRI), Yifan Cheng (UCSF), Wah Chiu (Baylor), Rado Danev (MPI), Joachim Frank (Columbia), Bob Glaeser (LBL), Steve Ludtke (Baylor), Chris Russo (MRC), John Rubinstein (Toronto), Sjors Scheres (MRC), Henning Stahlberg (U. Basel), Elizabeth Villa (UCSD), Tom Walz (Harvard).

The workshop is aimed at participants that already have some experience practicing cryoEM. We can accommodate a total of 100 participants. The workshop will be held at CUNY Advanced Science Research Center, 85 Saint Nicholas Terrace, New York, NY 10031, which is immediately adjacent to NYSBC where NRAMM is located.

A registration fee of $750 will be charged to participants from non-profit institutions. Most meals will be provided as part of the workshop registration fee but participants will be expected to cover their own travel and lodging expenses. Cost of housing is estimated to be less than $150/night for a shared room and transportation from hotels to the venue can be either by walking or using public transport ($2.75 each way). Further details on these and other matters will be provided at the Participants Guide

To be held at National Resource for Automated Molecular Microscopy, Simons Electron Microscopy Center, New York Structural Biology Center, New York, NY

The workshop can accommodate a total of 20 participants.

There are many challenges in preparing vitrified specimens of suitable ice thickness, and suitable particle preservation, distribution, and orientation, in a repeatable, reliable and robust manner. A major goal of the workshop will be to understand the issues underlying these challenges and discuss new approaches that will lead to improvements in all of these aspects.

Invited speakers include:
Bob Glaeser (LBL), Chris Russo (LMB/MRC), John Rubinstein (U. Toronto), Tom Walz (Rockefeller U.)

The application deadline is 17 March and a final selection of participants will be made by 27 March.

eBIC and CCP-EM are proud to announce the inaugural symposium of eBIC, the UK national cryoEM centre at Diamond Light Source, and the third CCP-EM Spring Symposium. This three day conference will be held at the Diamond/RAL Campus, Harwell, Oxfordshire.

The eBIC opening symposium will be on the first day, highlighting the advances of cryoEM and feature presentations with an emphasis on emerging and challenging technologies, followed by CCP-EM Spring Symposium featuring of state-of-the-art presentations related to cryo-EM methods and applications. Confirmed speakers include:

eBIC Speakers

Yifan Cheng, UCSF School of Medicine
John Briggs, MRC Laboratory of Molecular Biology
Lori Passmore, MRC Laboratory of Molecular Biology
Radostin Danev Max Planck Institute of Biochemistry
Greg McMullan, MRC Laboratory of Molecular Biology
Sharon Wolf, Weizmann Institute of Science

CCP-EM Speakers

Piet Gros, Utrecht University
Maya Topf, Birkbeck / UCL
David Waterman, STFC
Tanmay Bharat, MRC Laboratory of Molecular Biology / University of Oxford
Paul Adams, Lawrence Berkeley National Laboratory
Tristan Croll, University of Cambridge
Erik Lindahl, SciLifeLab
Kay Grunewald, STRUBI
Carolyn Moores, Birkbeck / UCL
Morgan Beeby, Imperial College London
Rob Nicholls, MRC Laboratory of Molecular Biology
Ausra Domanska, University of Helsinki
Becky Thompson, University of Leeds

Places are limited and registration will be on a first come first serve basis.

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Bridging the gap between cryo-EM and crystallography

AICS2017 "Bridging the gap between cryo-EM and crystallography" will offer
the opportunity to young researchers in biochemistry or structural biology
at the PhD/postdoctoral level to approach the fundamental questions arising
from the cryo-EM experiment setup. Central topics will include evaluation of
sample suitability for analysis and its preparation, choice of appropriate
instrumentation for single particle, tomography or electron diffraction
approaches, data processing and interpretation of results. A showcase of
recent results will contour the tutorial sessions.

Deadline for registration: 15 June 2017

The aim of the course is to teach the basic principles and practical
aspects of image processing to bioscientists and structural biologists
wishing to determine macromolecular structures by cryo electron
microscopy (EM). The course will concentrate on processing of single
particle images, and will be aimed at advanced PhD students and postdocs
working on projects producing cryo EM images for structural analysis.
Familiarity with UNIX would be an advantage.

The first half of the day will include two 50-minutes lectures and
discussions. A 3-hour practical would take place in the afternoon, with
discussion time at the end. After dinner there will be invited speaker
lectures or student presentations.

Applications are invited from PhD students and researchers in EM. Please
apply now via http://meetings.embo.org/event/17-cryo-em

The eighth edition of the Macromolecular Crystallography School is to be held at the Institute Química-Física Rocasolano - CSIC (Madrid).

 Invited speakers and tutors:

• Paul Adams, Berkeley, USA
• Pavel Afonine, Berkeley, USA
• Armando Albert, Madrid, Spain
• Roeland Boer, Barcelona, Spain
• Jose M Carazo, Madrid, Spain
• Kay Diederichs, Konstanz, Germany
• Paul Emsley, Cambridge, UK
• Rafael Fernández-Leiro, Cambridge, UK
• Carmelo Giacovazzo, Bari, Italy
• Xavier Gomis-Ruth, Barcelona, Spain
• Juan A. Hermoso, Madrid, Spain
• Eugene Krissinel, Oxford, UK
• José Martín-García, Arizona, USA
• Rob Nicholls, Cambridge, UK
• Maria Solá, Barcelona, Spain
• Isabel Usón, Barcelona, Spain
  

The workshop has been designed for postgraduate, postdoctoral and research scientists that deal with macromolecular crystallography but need further knowledge on the routine uses, the state of the art and fundamentals that underlie the modern techniques for macromolecular crystallography. The topics will cover crystallographic computing and fundamentals using XDS, CCP4 and Phenix, as well as the state of the art on topics such as the strategies for protein production, data collection,phasing and refinement with ARCIMBOLDO, SHELX and SIR2014 and modeling with COOT.

This year a full-day on breakthrough techniques such as XFEL and cryo EM has been introduced.

MCS2017 will provide six days of lectures and workshops. The course is designed for 25 highly motivated attendants.

Requests to participate must be sent by a free-formated E-mail, before March 10, 2017 to the following address: cursos@fgua.es, indicating the motivation to participate and experience on macromolecular crystallography and including a letter of recommendation from PhD Director (only for PhD students).

Accepted participants will be notified by e-mail before March 17, 2017.

Organizers: Armando Albert (xalbert@iqfr.csic.es), Juan A. Hermoso
(xjuan@iqfr.csic.es) and Isabel Usón ( iufcri@ibmb.csic.es).

Structure-Function Relations in Viruses and Virus-Like Materials