The course will take place in person at CNB-CSICÂ  
December 13 â€" 16, 2022 from 09.30-17.30 CET
 
The course aims at providing a comprehensive overview of the whole image processing pipeline in Electron Tomography (ET) from the movie alignment of the tilt series, 3D reconstruction of the tomogram, particle identification, and subtomogram averaging, all inside Scipion integration framework.

The course is aimed at researchers of all levels wanting to adopt electron microscopy as one of the tools at their disposal for elucidating biological structures.
 

Registration will be open until November 14, 2022

This 1-week course is focused on the theory and practice of cryo-ET methods. We will step through each stage of the cryo-ET workflow (specimen preparation, correlative light and electron microscopy (CLEM), cryo-ET data acquisition, and computational processing). The mornings will include lectures and stimulating round table discussions. The afternoons focus on hands-on practicals to reinforce the concepts and topics covered earlier in the day. Users are welcome to bring their own samples and/or data.

Remote course

June 22th to June 24th (2022) (3 days):
*3 Mornings: Presentations and training.
*2 Afternoons: Personal work.

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Hands-On Training Course that introduces the fundamentals and main tools needed to build an atomic model in the context of cryo-EM, starting from a 3D map and the aminoacid sequence of each protein chain. The tools will be used inside the Scipion framework (de la Rosa-Trevin et al. 2016) and examples will focus on the modeling by homology scenario, including AlphaFold2 prediction models.

Expected impact for young researchers

Atomic models based on high-resolution density maps are the ultimate result of the cryo-EM structure determination process. In this course, we introduce the basic tools needed to perform this task. Ph.D. students, as well as post-docs, will undoubtedly benefit from approaching this technique since atomic models guide our mechanistic interpretation of cryo-EM structures.

 Registration Fee: 50€

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A four-day VIRTUAL Cryo-Electron Microscopy

This course will introduce high-resolution Cryo-EM to the structural biology research community. It will include image formation and three-dimensional reconstruction theories and available software, tutorials, and demonstrations for data collection and analysis.

Confirmed speakers and instructors include:

David DeRosier, Brandeis University
Chen Xu, Umess
Tamir Gonen,UCLA
Gabriel Lander, Scripps Research
Oliver Clarke, Columbia University
Dongyan Tan, Stony Brook University
Jun Liu, Yale University
Digvijay Singh, UCSD
Jianfeng Lin, Yale University
Raphael Park, Yale University
Muyan Chen, Baylor College of Medicine
Yong Xiong, Yale University
Qun Liu,Brookhaven National Laboratory
Guobin Hu,Brookhaven National Laboratory
Liguo Wang, Brookhaven National Laboratory

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https://www.bnl.gov/cryoemcourse/files/pdf/agenda.pdf

Frederick National Laboratory will host a new training program in
Frederick, Maryland, September 12-16 for cancer researchers who want to
build expertise in cryo-electron microscopy. The workshop will cover topics
from grid preparation, imaging, and data processing. This workshop will be
in-person and attendees will have the opportunity to gain hands-on
experience with all stages of the cryo-EM workflow.

This six-days course is intended for PhD students and post-docs who expect to be using modern structural methods for future studies.

Registration deadline is May 2, 2022.

The Astbury Biostructure Lab (University of Leeds) is partnering with eBIC (Diamond light source) Scottish Centre for Macromolecular Imaging (University of Glasgow), Midlands Regional Cryo-EM Facility (University of Leicester) and Birkbeck college (University of London) to host training courses between 2021-2026 in cryoEM.
 
Training leads : Rebecca Thompson and Christos Savva 

The Astbury Biostructure Lab (University of Leeds) is partnering with eBIC (Diamond light source) Scottish Centre for Macromolecular Imaging (University of Glasgow), Midlands Regional Cryo-EM Facility (University of Leicester) and Birkbeck college (University of London) to host training courses between 2021-2026 in cryoEM.

This training course may be remote, but you will be able to get hands-on experience on Titan Krios microscopes as well as sessions on theory, sample preparation and on-the-fly data processing.

Subjecting matter to extreme conditions reveals a multitude of fascinating phenomena, and is applicable to a wide range of disciplines. From the extremophiles that exist in the deepest depths of our oceans to the exotic new materials that are made when atoms are pushed ever closer together, understanding the structure of materials at extreme conditions affects Biology, Chemistry, Physics, Geoscience and Material Science. In order to achieve its goals, high pressure research has been always at the vanguard of technical progress. It is a discipline in which equipment plays a particularly important role. State-of-the-art diamond anvil cells can now achieve megabar ranges of pressure, millions of times atmospheric pressure, with this beaten by an order of magnitude in dynamic compression experiments. In this manner, scientists are able to mimic the conditions in the interiors of giant planets. Nature is even more ambitious, as in the stars petapascal pressures (the order of tens to hundreds billion atmospheres) are reached. In the laboratory, we can even create conditions of pressure, temperature and magnetic fields that are not found naturally, allowing us to complement nature to explore more exotic phases of matter.

Therefore, the course will cover broad ground in the application of extreme conditions crystallography. The topics will span from fundamentals of high-pressure single crystal and powder diffraction, to presenting the many flavors of static and dynamic compression, to reviews of sources for extreme conditions work (synchrotrons, neutrons and free electron lasers). Complementary experimental (Mössbauer spectroscopy, X-ray magnetic circular dichroism, X-ray absorption spectroscopy, inelastic scattering) and computational methods will also be discussed. Diffraction analysis on the structure of liquids, glasses and nanocrystalline materials as well as multigrain crystallography will also be introduced. The lectures will be harmonized with the related sessions of interactive tutorials, providing hands-on experience to the attendees. These workshops will be focused on basic laboratory skills and the elements of data processing software related to the high pressure.

We anticipate holding the course in person as long as the pandemic situation continues to improve. Appropriate COVID-19 precautions will be followed to ensure the safety of all participants. Everyone will be required to provide documentary proof of a prior FDA or EMA approved vaccine.

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This immersive course is an outstanding place to learn both the theoretical and practical aspects of Macromolecular Crystallography because of the extensive lectures from world-renowned teachers, which are combined with hands-on experiments. This 2021 course will be lead by Jim Pflugrath (Rigaku, ret.), Tassos Perrakis (NKI), Paul Adams (LBL), Janet Newman (CSIRO; likely remote)), Tom Peat (CSIRO; likely remote) and an All-Star cast of lecturers. Attendees can expect to participate in a course that is unparalleled in the world, with experts each devoting several days to teaching the fundamentals, theory and practical considerations of crystallographic structure solution.

Topics to be covered include

• Basic diffraction theory
  
• Crystallization (proteins, nucleic acids, complexes and membrane proteins)
  
• Synchrotron X-ray sources and optics
  
• Data collection and processing
  
• Structure solution by experimental phasing methods (SAD, MAD, MIR, and others) and molecular replacement
  
• Electron density maps improvement
  
• Model building and refinement
  
• Structure validation
  
• Coordinate deposition
  
• Structure presentation

The course is limited to 16 participants due to the very hands-on nature of the experiments and the intimate seminar room and laboratory settings. Please check the above web link for more details. In particular, please note the information about fellowships, scholarships, and stipends that are available. It is often possible for applicants to receive some level of support to help offset the course fee.

The aim of the course is to provide in-depth understanding of theory and practice in cryo-EM image processing, covering basic topics such as Fourier Transforms, image formation and CTF, to more advanced ones such as Bayesian methods, subtomogram averaging, postprocessing, high resolution refinement and atomic model building.

A series of pre-recorded lectures will be provided to watch in advance, and during the course there will be live video Q&A sessions with the experts. These will be followed by practicals on a wide range of commonly used software. The practicals will mainly be led by the software developers, giving a unique chance to understand in depth the ?black boxes?. In addition, we will have exciting live sessions and social interactions.

We will only be able to take a very limited number of applicants, who will be selected based on a series of required criteria:

skills and motivation:

- some existing level of exposure to cryo-EM
- basic familiarity with linux/command line operation
- a commitment to continuing work with cryo-EM and to spreading the knowledge locally.

technical requirements:

- a fast and stable internet connection
- ideally two monitors (or one plus a tablet: practicals will run on parallel guacamole and zoom sessions)

Applications will only be open for a short time (closing in early July); we are sending this message now so that you can gather all the necessary documents in advance. The application form will require a cover letter, a CV, and a letter of recommendation from your supervisor. Please make sure it is clear in these documents how you meet the above criteria. An email with the details on how to apply and the necessary links will be sent to this mailing list as soon as possible.

We will take participants from PhD level up, and we ask that only one application is sent from any institution.
 
https://meetings.embo.org/event/21-cryo-em 

The remote practical course covers principles, differences and applications of macromolecular crystallography using X-rays, neutrons and electrons. The course is targeted to PhD students and postdocs whose research project focuses on solving structures using crystallography.

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Teachers:

Eugene Krissinel (CCP4, UK)

Andrey Lebedev (CCP4, UK)

Esko Oksanen (ESS, Sweden)

Edward Daniel (University of Oulu, Finland)

Bernhard Rupp (Medical University Innsbruck, Austria)

Lectures: June 21 - June 25, 2021

Tutorials: June 28 - 30, 2021

2:00 pm - 5:30 pm EST

Registration (free) is now open and will close May 14, 2021: https://conference.sns.gov/event/291/registrations/
 

This online course is designed for graduate students, post-doctoral researchers and faculty with knowledge of structure and function of biological macromolecules but with limited to no experience with neutron sciences. The course will cover the basics of deuterium labeling, small angle neutron scattering, neutron reflectometry, neutron spectroscopy, neutron imaging and neutron crystallography. Lectures will cover basic concepts, sample preparation, instrumentation and applications. Data collection and processing will be discussed in tutorials.

Over the years, many techniques and procedures have been developed that greatly enhance the versatility of the method. The purpose of the X-ray clinic is to combine theoretical and practical application of X-ray fluorescence spectrometry.

The clinic is presented in a week-long session and is directed to both relative newcomers in the field and to more experienced users wishing to broaden their understanding of fundamental concepts and established procedures. The major emphases are the acquisition of reliable experimental data and qualitative phase identification using manual search techniques, and computer-based methods of qualitative and quantitative phase analysis.

This clinic introduces the theory and formalisms of various indexing methods and structural refinement techniques. One unique aspect of this clinic is the extensive use of computer laboratory problem solving and exercises that teach method development in a hands-on environment. We will use EXPGUI and GSAS for the Rietveld laboratory work; Crysfire (especially Dicvol, Ito and Treor) will be used for indexing exercises.

Advanced Methods in X-ray Powder Diffraction, is designed for the experienced user and focuses on computer-based methods of qualitative and quantitative phase analysis, as well as crystal structure analysis and refinement. For maximum benefit from the Advanced Methods in X-ray Powder Diffraction, the ICDD Clinic faculty strongly recommends that students have previously attended Session I. The format of the clinic is a combination of morning lectures and afternoon recitations and workshops.