9:00 AM - 6:30 PM on 11 Nov, 9:00 AM - 2:00 PM on 12 Nov AEDT

Â

This webinar will be presented by Claudio Catalano, a senior scientist at NanoImaging Services.

Â

At 11am PST / 2pm EST. Registration is FREE

Time: Thursday, June 27, 2024 at 9 AM pacific / 12 PM eastern time

Speaker: Dr. Keerthi Gottipati, Indiana University, Bloomington

Title: Cryo-EM structure of HIV-1 Rev Response Element RNA: Structural basis of Rev-RRE interaction
 

All are welcome to attend. Registration is at no-cost, but sign-up is required:

This 5-day course is largely aimed at structural biologists with EM maps suitable for modelling building and refinement. This course will host some of the leading software developers and provide ample contact time to allow delegates to discuss their data in detail alongside traditional lectures and tutorials. This is a comprehensive course for EM model building covering advanced use of LocScale, ModelAngelo, Buccaneer, findMySequence-checkMySequence, EM_placement, Coot/Moorhen, TEMPy-REFF, ISOLDE, Refmac-Servalcat, Privateer, new validation tools and AlphaFold-DB & EMDB/PDBe updates. It will cover all aspects of modelling building including: map optimisation, automated model building, model fitting, medium resolution refinement, high resolution refinement, interactive refinement, validation and deposition.
 

* Confirmed tutors / topics:

Tom Burnley (CCP-EM) : CCP-EM Doppio
Arjen Jakobi (TU Delft) : LocScale
Sjors Scheres (MRC-LMB) : Relion5 and ModelAngelo
Randy Read (University of Cambridge) : EM-placement
Grzegorz Chojnowski (EMBL) : FindMySequence/CheckMySequence
Maya Topf, Joseph Beton and Aaron Sweeney (CSSB Hamburg) : TEMPy-REFF, Chem-EM
Judit Debreczeni (Astrazeneca), Lucrezia Catapano (MRC-LMB) and Paul Bond (University of York): Coot / Moorhen
Soon Wen Hoh and Paul Bond (University of York) : Buccaneer/ModelCraft
Rob Nicholls (CCP4) and Rangana Warshamanage (CCP-EM) : Refmac/Servalcat
Tristan Croll (AltosLabs) : ISOLDE
TBC (University of York) : Privateer
PDBe (EBI) : AlphaFold-DB and 3D-Beacons
Agnel Joseph (CCP-EM) : Model validation
Kyle Morris (EBI) : EMDB deposition and validation

Registration of interest will close either when all places are taken or by Friday 12th July 2024

The Applied Crystallization Workshop at the National Crystallization Center at HWI in Buffalo, NY, USA.  Does your macromolecular crystallization project feel like the Wild West? Join our two-part Crystallization Workshop to learn to wrangle your crystals!

What is it? A macromolecular crystallization workshop

• Two separate 1.5 day on-site components in June OR August 2024 (apply here:https://forms.gle/y1BYJazpY5gsMoj9A) Â

Who is it for? Open to researchers at all levels: students, postdocs, PIs

Favored applicants for on-site workshop will have submitted samples for high-throughput screening prior to the virtual workshop and have a sample to bring to set up.

Deadlines:

Applications for the August 20-21 workshop must be submitted by July 8th, 2024

For the on-site portion of the workshop, applicants must be available to travel on-site to Buffalo, New York during the dates of August 20-21, 2024. On-site workshop spaces are limited and applicants will be selected based on suitability of project and application. Note some travel support is available for researchers at US-based universities.

Workshop supported by NIH R24GM141256. We gratefully acknowledge the workshop sponsors MiTeGen, Formulatrix, SPTLabtech, and Hampton Research.

The Applied Crystallization Workshop at the National Crystallization Center at HWI in Buffalo, NY, USA.  Does your macromolecular crystallization project feel like the Wild West? Join our two-part Crystallization Workshop to learn to wrangle your crystals!

What is it? A macromolecular crystallization workshop

• One day virtual component on May 23 2024 (register here:https://us06web.zoom.us/meeting/register/tZclfuGpqDgvHNZNhBxWgGA2wT4zdd5AuDsW)Â

• Two separate 1.5 day on-site components in June OR August 2024 (apply here:https://forms.gle/y1BYJazpY5gsMoj9A) Â

Who is it for? Open to researchers at all levels: students, postdocs, PIs

Favored applicants for on-site workshop will have submitted samples for high-throughput screening prior to the virtual workshop and have a sample to bring to set up.

Deadlines:
Applications for the June 20-21 workshop must be submitted by May 6th, 2024
Applications for the August 20-21 workshop must be submitted by July 8th, 2024

For the on-site portion of the workshop, applicants must be available to travel on-site to Buffalo, New York during the dates of June 20-21, 2024 OR August 20-21, 2024. On-site workshop spaces are limited and applicants will be selected based on suitability of project and application. Note some travel support is available for researchers at US-based universities.

Workshop supported by NIH R24GM141256. We gratefully acknowledge the workshop sponsors MiTeGen, Formulatrix, SPTLabtech, and Hampton Research.

The aim of this course is to help researchers get introduced to the area 
of flexibility/heterogeneity analysis, one of the most advanced topics 
in SPA. We will present the theory behind some of the most used methods 
in the field (some developed by us, some by others) and conduct short 
practical sessions. These sessions will take place within the 
integrative Scipion Flexibility Hub environment to assist in the use of 
these multiple methods.

Second edition of the Grenoble Practical Workshop dedicated to helical symmetry determination of biological polymers imaged by cryo-EM.

Please note that this year, due to the specific procedures required, we regret to inform you that projects involving *amyloid* or *microtubules will not be accepted*.

Determining the helical parameters remains a significant challenge for many researchers, and our workshop aims to provide hands-on guidance through this intricate process. We will delve into power spectra interpretation, 2D class-averages analysis, and raw data inspection to extract valuable insights.

While helixplorer will be a key tool covered in the practical sessions, we encourage exploration beyond this approach to accommodate diverse research needs.

Due to limited space, in-person places are strictly limited to 12 delegates:

 7 spaces are reserved for our internal DTP Students (attendance by invitation) and are funded by the Wellcome Trust

 5 spaces are available by application to selected PhD students from the community (application details below) - these spaces are available for non-UK based PhD students and are funded by iNEXT-Discovery

The Polish Crystallographic Meeting, Scientific Workshop and Session of the Young Crystallographers Sections of PCrA will be held at the Institute of Low Temperature and Structure Research of the Polish Academy of Sciences, ul. Okólna 2 in Wrocław.

The Workshop is devoted to selected issues and problems of modern crystallography.

The Scientific Session of the Young Crystallographers Section of PCrAÂ is a forum for exchanging experiences addressed primarily to students, PhD students and young doctors (maximum 5 years after defense).

The topics of the Meeting include basic and applied research on the ideal and real structure of crystals carried out with the use of X-ray radiation obtained both with traditional methods and in synchrotrons, research with the use of neutrons and electrons, problems of symmetry, phase transformations and crystal growth, new research and computational methods and all other aspects of crystallography. The conference is an annual meeting place and a forum for exchanging ideas of all Polish crystallographers.

 
Confirmed speakers and instructors include:

• Ben Barad (OHSU)Â
• Muyuan Chen (Stanford)Â
• Oliver Clarke (Columbia)Â
• David DeRosier (Brandeis)Â
• Wim J. Hagen (ThermoFisher)Â
• Guobin Hu (BNL)Â
• Jake Kaminsky (BNL)Â
• Jianfeng Lin (Yale)Â
• Qun Liu (BNL)Â
• Jun Liu (Yale)Â
• Mike Martynowycz (HWI)Â
• Dongyan Tan (SBU)Â
• Liguo Wang (BNL)Â
• Fred Sigwroth (Yale)Â
• Yong Xiong (Yale)

Course Topics

• Introduction to LBMS
• Introduction to electron microscopy and structural biology
• Introduction to electron microscope and image formation process
• Introduction to negative staining and cryo-electron microscopy
• Single-particle sample preparation tutorial and demonstration (negative staining and cryogenic vitrification)
• Single particle reconstruction: theory, application and available software
• MicroED: theory, application and available softwareCryo-electron tomography
• EPU single particle data collection tutorial and demonstration
• Model building, refinement, and validation.
• Cryo-FIB sample preparation tutorial and demonstration
• SerialEM electron tomography data collection tutorial and demonstration
• Single-particle data analysis workflow tutorial and demonstration
• Tomographic data analysis workflow including subtomography average tutorial and demonstration

The full schedule can be found at https://www.bnl.gov/cryoemcourse/files/pdf/agenda.pdf 

The timing of this event is such that attendees have the option to continue on to the Asia-Pacific Microscopy Conference in Brisbane (February 2-7), where there will be a substantial cryo-EM program. Other options include the Lorne series of conferences.

The symposium celebrates the Japanâ€"UK partnership and brings together wide range of experts from Japan covering all of the frontier structural biology approaches including cryo-electron microscopy, synchrotron and free-electron laser X-ray crystallography and neutron crystallography.

The symposium will have two full days of lectures, the first day finishing with a public lecture by the Nobel Laureate Richard Henderson and the second day with lectures (open to the public) from Sir Tom Blundell and Dr Harren Jhoti, FRS (President and Chief Executive of Astex Pharmaceuticals).

The symposium is supported by the University of Liverpool and RIKEN together with BBSRC, STFC and ILL.

Registration is essential and is limited to 125 delegates. A small registration fee of £50 is being set to encourage ECR and PDRAs participation.

See the programme here.Â

Engineered Protein Cages for Imaging of Small Proteins by Cryo-EM by Dr. Roger Castells-Graells at 12pm Eastern time.