Fundamentals and Practices of Macromolecule Crystallography Using Synchrotron Light: From Protein Crystallization to Structural Determination

This four-day workshop aims to provide theoretical and practical training in the foundations and advanced techniques of protein crystallography. Among the main topics covered are protein crystallization, macromolecular X-ray crystallography (MX), data collection and processing, and new emerging trends in structural biology.

The third edition of the Grenoble practical workshop focused on the determination of helical symmetry in biological polymers imaged by cryo-EM will take place online from 
July 21st to 25th, 2025.
Ed Egelman and Arjen Jakobi will be speakers, whose expertise will greatly enrich both our discussions and hands-on sessions.

While *Helixplorer* (https://urldefense.com/v3/__https://rico.ibs.fr/helixplorer/__;!!Mih3wA!E8k6u4PapE61RoHy1fNa_JmGof9e65JNpZs2Dcf7d1ogbvN9gxduVMX-fpPxMI-nzq_qTqbseDr52Jf6kB_Jv66N9Rjr$ ) will play a central role in our practical sessions, we also encourage participants 
to explore alternative approaches to address a variety of research needs.

Beyond helical parameter determination, we will also explore `broader 
reconstruction strategies', including managing flexible helices, working 
with multiple symmetries, and integrating data from different filament 
symmetries using subtraction techniques.

We welcome applicants at all career stages (PhD students, postdocs, 
senior scientists, and beyond) who are currently working on helical 
samples and facing structural analysis challenges. During the workshop, 
participants will use their own data, sharing selected raw images, 2D 
class averages, and power spectra with the organizers. The specific 
identity and function of your protein or filament will remain confidential.

To apply, please email your application?including a brief description of 
your project and the difficulties you?re encountering, as well as 
screenshots of your raw data and 2D class averages with the 
corresponding *pixel size*? to:

 *ambroise.desfosses@ibs.fr*

 *leandro.estrozi@ibs.fr*

*Application deadline: June 10th.*

The workshop will offer a synergistic combination of lectures and practical hands-on sessions supported by leading experts in the field, providing the participants with the unique opportunity to establish a broad network with international specialists in the field.

This course will enable the participants to: prepare thick biological specimen using high-pressure freezing for on-grid lamella preparation (waffle method), implement cryo-CLEM correlative workflows for 3D targeting, set up automated lamella preparation, and perform cryo-volume imaging data acquisition.

We bring together experts at the intersection of microscopy and mass spec, topics include soft landing, holography, cryoEM, AFM, native MS, top-down proteomics and various single molecule imaging and mass spectrometry techniques to characterize (biological) macromolecules.

An Institut Pasteur -Synchroton SOLEIL scientific meeting
Practical trainings will follow the days after (July 1 â€" July 4) for the
PhD candidates of the Marie Curie Doctoral Network on both Soleil and 
Pasteur campuses. These will involve Cryo-confocal 
Stellaris/super-resolution STED, Cryo-Electron Tomography, XRD, and 
Cryo-Interferometry.
 
Registration is now open and free of charges (but mandatory). Please register by June 15, 2025.

• Â Â Â AlphaFold Training: Master the use of AlphaFold for accurate protein structural determination.
• Â Â Â Drug Discovery: Learn to use protein structures (AlphaFold2 and experimentally solved structures) to screen small molecule compound libraries.
• Â Â Â Structural Analysis: Participate in practical sessions on protein structural analysis and interpretation.
• Â Â Â Expert Lectures: Gain insights from leading professionals in the field.
• Â Â Â Networking: Build connections with peers and experts.

At 4 pm CET

Lecture 47: Quantum Crystallography for Macromolecular Systems: applications and recent developments given by Benoit Guillot (Université de Lorraine, CNRS, Nancy, France)

Lecture 48: Optimal Clustering for Quantum-Based Refinement of Biomacromolecules given by Malgorzata Biczysko (University of Wrocław, Poland), Holger Kruse (Pending AI Pty Ltd., Australia), Nigel W. Moriarty(LBNL, Berkeley, USA), Mark P. Waller (Pending AI Pty Ltd., Australia) and Pavel V. Afonine (LBNL, Berkeley, USA)

The University of Liverpool will be bestowing an Honorary doctorate to Dr Richard Henderson.  To mark this, a Celebratory symposium entitled “cryoEM revolution in structural biology’ has been organised at the University of Liverpool.Â

This one-day Symposium will bring speakers from the Universities of Leeds, Liverpool, Sheffield, Imperial and Birkbeck as well as from the Crick Institute and Astex Pharmaceuticals.

Dr Henderson has received several prizes including the Gregori Aminoff prize for Crystallography from the Royal Swedish Academy (1999); Copley Medal from the Royal Society (2016); Gjonnes medal in electron crystallography from the International Union of Crystallography (2017), Nobel Prize for Chemistry (2017) and Companion of Honour (2018).

The conference was launched in 2018 to bring the industry-based structural biology community together for two days of presentations and networking. This is a great place for industrial structural biologists, as well as those who are industry-curious.

The conference will feature keynote presentations by:

* Dr. Stephen K. Burley, Director RCSB Protein Data Bank and Rutgers University Professor

* Dr. James Fraser, Professor and Chair Department of Bioengineering and Therapeutics Sciences at UCSF

* Dr. Stephen Harrison, Professor Harvard Medical School/Investigator Howard Hughes Medical Institute

* Dr. Mark Murcko, Founding CSO/Board Member at Dewpoint Therapeutics and Relay Therapeutics

 

Scientific sessions will cover the following topics:

* Structural Biology of Targeted Protein Degradation: Glues, TCs & DUBs

* Developing Fragments to Drugs: Challenges & Victories

* Molecular Dynamics and Allostery - Bridging Experimental and Computational Drug Discovery

* Membrane Protein Structure Guided Design

* CryoEM Synergies with Molecular Modeling and Computational Models

* Biologics (Antibody Design & ADCs)

* AI/ML in Structural Biology and Drug Discovery

* Hot Topics (SBDD, Kinases, RNA)

• Studies of dynamic systems, 4D structural biology
• Electron and X-ray tomography, and correlative approaches
• Microcrystal Electron Diffraction of Proteins & small molecules
• Neutron Diffraction and Scattering for biological macromolecules
• From structure-based drug discovery to clinical trials
• Integrative approaches in Structural biology
• AI in structural biology, software and databases
• EPN research institutions and facilities

This semi-annual workshop will focus on practical aspects of single 
particle cryo-EM, including negative staining and cryo-sample 
preparation, screening, data collection, and analysis using the LBMS 
sample preparation room, electron microscopes, and GPU workstations.

To optimize interactions and hands-on experience, this workshop will be 
limited to 16 participants.