Hosted by CCP-EM and the Astbury Biostructure Laboratory (University of Leeds, UK) this one day workshop will focus on the RELION software package and is led by its developer Sjors Scheres (MRC-LMB).

The training will involve a hands-on tutorial of RELION 2.0 for cryo-EM single-particle analysis (http://www2.mrc-lmb.cam.ac.uk/relion). It will cover the entire process from initial micrograph inspection, CTF estimation, (automated) particle picking, 2D and 3D classification, 3D high-resolution refinement, movie-processing, and map sharpening.

Computational facilities will be provided along with refreshments.

Registration is free but not a guarantee of acceptance. Application for registration will close on Monday 15th August 2016, participants will be selected and we will confirm places no later than Friday 19th August 2016. There are 30 places at the workshop.

To address the recent surge of interest in the general biophysical community, the Cryo-EM Subgroup was initiated at the 2016 meeting with a program of speakers that were
selected from submitted abstracts. For the 2017 meeting, we have invited
the following speakers:

• Gira Bhabha, Univ. of California, San Francisco
• Eric Gouaux, Vollum Institute, Oregon Health & ScienceUniv
• Pascal Krotee (David Eisenberg Lab), Univ. of California, Los Angeles
• Rod MacKinnon, Rockefeller Univ.
• Stefan Raunser, Max Planck Inst of Molecular Physiology, Dortmund
• Kliment Verba (David Agard Lab), Univ. of California, San Francisco

We anticipate that the Cryo-EM Subgroup will meet on Saturday evening
February 11, 2017; this time slot will enable you to also attend other
subgroups earlier in the day. Other Cryo-EM sessions at the meeting
include a Workshop as well as a Platform Session with speakers selected
from submitted abstracts. To view the entire program for the 61st
meeting, you should visit the recently launched website, which will be
accepting abstracts up until the October 3 deadline:
https://www.biophysics.org/2017meeting. Be sure to consider the Cryo-EM
category (10B) when submitting your abstract.

Finally, when you register for the 61st meeting, we very much encourage
you to join the Cryo-EM subgroup. The associated fee goes directly
towards supporting our program.

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The 8th International Conference of the Hellenic Crystallographic Association (HeCrA) and the 11th Conference of the Hellenic Society for Computational Biology and Bioinformatics (HSCBB) are jointly organized as HeCrA-HSCBB16.Â

HeCrA-HSCBB16 will take place at the Agricultural University of Athens in October 7-9, 2016.Â

The conference will address key questions and issues in molecular structure and interactions. The Conference is the major event both of HeCrA & HSCBB. It is a forum to communicate state-of-the-art research, interact and establish scientific networks and collaborations.

In particular, it will bring together crystallographers with Bioinformaticians and Computational Biologists for the identification of macromolecular targets and small molecules of pharmaceutical and biotechnological interest and study their interactions.

There will be oral and poster presentations in addition to talks from distinguished invited speakers from Greece and the European Union.Â

Organisers: Hellenic Crystallographic Association & Hellenic Society for Computational Biology and Bioinformatics.

Organized by the Diamond Light Source Macromolecular Crystallography group.

The aim is to provide MX users with sufficient training to be able to operate any of the Diamond MX beamlines efficiently and to get the most benefit from their beam-time. The training will involve hands-on sessions on the suite of five operational MX beamlines (http://www.diamond.ac.uk/mx-home/) as well as optional offline software sessions.

20th July afternoon optional session "Hands on software":

• CCP4
• DIALS

21st July, MX beamlines and data room sessions:

• MX software: automation in data analysis
• Mini-Kappa goniometry / Anomalous data collection
• Introduction to VMX-i
• I24 new end station/ In-situ diffraction
• Fragment screening â€" I04-1/Lab36
• Experiment database: new ISPyB

Registration is free-of-charge with lunch provided on the 20th and 21st July, and accommodation and dinner for the night of July 20th.

June 15th deadline for applications

MicroED is a cryo-EM method that uses electron diffraction to facilitate structure determination from sub-micron sized 3D crystals of biological material. MicroED uses equipment and procedures that are standard on many cryo-EM:s, and because of this the technique can be easily adopted in labs and facilities around the world. To encourage the widespread use of MicroED we are offering this workshop to teach microscopists how to perform the technique and adopt it at their own institutions. The first MicroED workshop was held in February of 2016 and was heavily oversubscribed. For this reason we are offering a second workshop.

There is no registration fee for participation, and Janelia will provide on-site housing and meals. Attendees are expected to cover their own travel expenses and must be able to stay for the duration.

Space in the course is limited. The application deadline is August 1, 2016 (11:59 PM EDT)

Ouverte à tous ceux qui souhaitent s'initier ou approfondir leurs connaissances en modélisation 3D, docking - criblage virtuel, dynamique fonctionnelle, mécanique quantique et visualisation 3D de molécules biologiques. L'école sera divisée en sessions théoriques et pratiques pour mieux appréhender toutes ces méthodologies. L'objectif est de repartir avec une maîtrise des différents outils logiciels disponibles dans ce domaine, qui sont le plus souvent, en libre accès pour la communauté scientifique.

Les grandes lignes du programme sont:
Analyse des séquences pour la modélisation 3D (bases de données Pfam, UniProt,...) Énergie libre, entropie et perturbation (FEP) Modélisation moléculaire par homologie et Ab initio (Modeller, Robetta,...) Conception de molécules pour le Drug Design (Chimiothèques, PubChem,...) Docking / Scoring et criblage virtuel de chimiothèques (AutoDock Vina,...) Simulations de dynamique moléculaire, QM/MM et modes normaux (Amber, Gromacs, NAMD,...) Visualisation 3D et dynamique interactive (VMD, PyMol, Chimera, UnityMol,...)
Toutes les informations disponibles sur le site: https://modyn.sciencesconf.org/
ATTENTION, le nombre de place est limité - Date limite de dépôt des candidatures: 30 juin 2016 Contact: modyn@sciencesconf.org

This Workshop follows on from the previous two workshops at NIST in February 2014 and Diamond in October of 2014 and the interim closed project meeting held at SAS-2015 in Berlin in September 2015. The Workshop will consist of three parts. On Monday will be the presentation sessions of broad interest to the scientific community. Attendees are encouraged to bring posters explaining their use of these kinds of modeling techniques or illustrating their need for such. Tuesday morning will be devoted to a meeting between the CCP-SAS project and individuals or institutions with activities in related software development areas, interests in participating in the CCP-SAS current or projected activities and facilities with potential interest in deploying CCP-SAS products on their servers for use by their users and thus have a potential interest in helping with maintenance activities. Discussions will focus on current activities, directions and needs with a goal of elucidating adjustments to CCP-SAS activities in the remaining funding cycle, and, more importantly of developing collaborations with other projects and identifying areas for joint funding applications moving forward. Finally on Wednesday there will be two hands on tutorial sessions: How to wrap existing code using GenApp to produce a web GUI and run on a cluster backend, and code development in the SASSIE framework.

The workshop will provide hands-on training on practical aspects of 2D crystal data collection on a Titan Krios, and cover in depth the theoretical foundations and the practical image processing of 2D crystal cryo-EM images with the 2DX software package. No previous experience is required.

Topics include:

Basics of 2D electron crystallography

• Introduction to 2D electron crystallography
• Cryo-electron microscopy sample preparation for 2D crystals of membrane proteins
• Cryo-EM data collection of 2D crystals
• Introduction to 2DX
• Drift correction using ZORRO Â

Basic image processing with 2DX

• Determination of the defocus, crystal tilt geometry, crystal lattice
• Crystal Unbending - including movie-mode drift correction of individual unit cells in dose-fractionated image series
• 2D merging and 3D merging with reconstruction via weighted back projection
• Automation of the processing pipeline

Advanced topics in image processing with 2DX

• Approaches for a partial reconstruction of amplitudes and phases inside of the missing cone
• Processing of 2D crystal unit cells with RELION and FREALIGN, under exploitation of neighborhood correlation of certain image parameters
• Advanced usage of the 2DX backend library
• Preparation of final results with quantitative description of statistical values (e.g., resolution) for export to databases (EMDB).

Computing infrastructure will be provided in place and participants are welcome to bring their own data sets and/or computer (OSX or Linux).

For registration, please include information about your affiliation, and a short motivation statement (max 200 words).
The registration fee is 250 CHF (travel and accommodation is not included), registration deadline is June 30th.

Fifth Dynamo workshop

The workshop will provide hands-on training on all practical aspects of sub-tomogram averaging, encompassing all stages of the pipeline: tomogram visualization and archiving, particle picking in different geometries, averaging, refinement and classification. No previous experience is required. Computing infrastructure will be provided in place and participants are welcome to bring their own data sets.

The course is intended primarily for post-doctoral researchers and Ph.D. students in structural biology with at least one year’s relevant experience in 3D-structure determination of macromolecules (X-ray diffraction, SAXS, cryo-electron microscopy). The workshop will cover state-of-the-art methodology for data processing with emphasis on integration of different methods to solve structures of macromolecular complexes and ‘difficult’ targets. The courses will cover theoretical and practical aspect of:

• X-ray data processing (diffraction and SAXS)
• Electron microscopy data processing
• Phasing, refinement and validation (structure and data)
• Integration of data from different experimental sources (X-ray diffraction and diffusion, cryo-electron microscopy), structure prediction to solve difficult problem (macromolecule reticent to crystallization, large complexes)
• Ongoing and future developments (methods, techniques and instruments) for integrated structural biology.

This training course will contain full lectures and practical sessions. Full classes will be done in the morning sessions and practical will be organized each afternoon in subgroups that will be rotating. Participants may bring their own X-ray and cryo-electron microscopy data. Data collection and processing of solution structure by SAXS or X-ray diffraction will be available using the INSTRUCT CENTER - FRANCE 1 â€" IGBMC platforms. A visit of the Center of Integrated Structural Biology of the IGBMC will be included in the workshop program.

The number of participants is limited. 25 candidates will be selected by a committee (see Website for registration details and application). No registration fees for the workshop.

The workshop will be couple to the meeting "Current Challenges in Integrated Structural Biology" (October 3 - 4, 2016, IGBMC). A web site will be open shortly for the meeting. We strongly encourage participants of the workshop to attend the meeting.

This workshop forms part of the Instruct and of the FRISBI Training Course Programme 2016. It also part of the French national PhD program in Integrated Structural Biology (ReNaFoBiS).

On 6th June there is an option workshop: Quantitative Phase Analysis by XRPD: organizer Arnt Kern (arnt.kern@bruker.de); instructors Arnt Kern and Fabia Gozzo (fabia.gozzo@excels.us)

The BCA/CCP4 Protein Crystallography Summer School is intended for students and researchers new to crystallography. Its dual aims are to provide comprehensive training in crystallography and to promote best practise within the British crystallographic community. The lectures cover the gamut of techniques required for a structure solution, from protein expression through to structure validation. Intensively supervised computer tutorials enable students to relate the theory presented in the lectures to practice. Although CCP5 programmes will primarily be used in these tutorials, the aim will be to approach crystallographic questions in a software-agnostic manner. There will also be a crystal manipulation and cooling workshop. (Note that the course is scheduled during Diamond synchrotron downtime, so there will not be active data collection at the Diamond beamlines.)

We recognise that an active social programme plays a role in enabling students to make links that may be maintained throughout a research career. There will be a conference dinner, and a number of other events, including a boat trip on the Thames from Oxford.

The proceedings are streamed live at https://livestream.com/fccollege/.

This workshop focuses on motion of biomolecules (proteins, nucleic acids, complexes) which occurs as they perform their functions. "Motion" includes conformational changes, ranging from large domain "hinging" motions to relatively small loop motions (e.g. to allow and deny access to an active site); oligomerization changes; interaction with partner molecules.

MacCHESS is offering its sixth intensive HOWTO course in BioSAXS. Students will have a day and a half of lectures and hands-on software tutorials on the basics of BioSAXS data collection and processing from expert practitioners in the field. This will be followed by real data collection on MacCHESS beamlines (F1 and G1 stations).

While the primary purpose of the course is educational, students may bring a limited number of their own research samples. Prepared standard protein samples will be available to all students for practice.

This course now accepts remote students. These students will receive digital copies of all lectures, tutorials, and sample data, and will have access to a live stream of the course lectures. Remote students will not have data collection time during the course. This option is intended for current MacCHESS BioSAXS users who want a refresher, or for new users who have scheduled beamtime this May but cannot come to CHESS twice.

Course topics:

• Â Basic principles and processing methods
• Â Critical sample preparation and data collection procedures
• Â Evaluating data quality
• Â Computing envelopes and data modeling
• Â Online size-exclusion chromatography (SEC-SAXS)
• Â Overview of advanced and emerging methods
• Â What you need to know for publication

An Inaugural Symposium at UT Southwestern

Distinguished guest speakers include the cryo-electron microscopy and tomography experts Yifan Cheng (UCSF), Wah Chiu (Baylor College of Medicine), Nikolaus Grigorieff (Janelia Research Campus) and Dick McIntosh (University of Colorado, Boulder).